Part:BBa_K4601005

NpSRII

This part is the NpSRII sensory rhodopsin.

Usage and Biology

NpSRII is a sensory rhodopsin identified in the archaea Natronomonas pharaonis.

Sensory rhodopsins are not pumps, but photoreceptors that respond to light and transmit the signal into the cell via a signaling cascade. For this, in N. pharaonis, NpSRI and NpSRII interact with the transducer proteins NpHtrI and NpHtrII, respectively [1]. NpHtrI and NpHtrII are homodimeric membrane proteins that have also a cytoplasmic histidine kinase domain responsible for initiating the signaling cascade that mediates chemotaxis. The interaction between the SR and their cognate Htr transducers are highly specific.

For the characterisation of this part an expression cassette was assembled and the results are presented on its page in Parts Registry: BBa_K4601205.

References

[1] Jung KH, Spudich EN, Trivedi VD, Spudich JL. An archaeal photosignal-transducing module mediates phototaxis in Escherichia coli. Journal of Bacteriology (2001) 183: 6365–6371.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 73
  Illegal NgoMIV site found at 331
  
• 1000
  COMPATIBLE WITH RFC[1000]